Table 1 Primers used in the quantitative Real time PCR (qRT-PCR)
From: Phylogenetic, syntenic, and tissue expression analysis of slc22 genes in zebrafish (Danio rerio)
Protein name | Primer sequence 5′ -> 3′ | Ta | Final conc. (nM) | Efficiency (%) |
|---|---|---|---|---|
Oatl | F TGCTGTTCTGATCTTGGACGA | 62 | 300 | 92 |
R TGCT ATTAAACCAGCGAT GAC | 60 | 300 | ||
Oat3 | F GGGTCAGCATTTACCTCATCCA | 60 | 300 | 105 |
R GATGGCCGTCGTCCTAACAT | 58 | 300 | ||
Oat2a | F TCGCCATTGCAAGAACCTTAT | 58 | 300 | 92 |
R AAGGTGCGATGCTTAACATCTG | 58 | 300 | ||
Oat2b | F GATTGTAAGTGTTCCAGCACAAGAA | 58 | 300 | 101 |
R TGAGCTGCTGGACGAGTTTATC | 58 | 300 | ||
Oat2c | F GCACTTTGATAACAGCACCTTCAT | 58 | 300 | 95 |
R GAAGAAGATGGTGGTTGTCAATTTC | 59 | 300 | ||
Oat2d | F ACAGTATGGCATGGGCTGTT | 60 | 300 | 100 |
R AAGGTGAAGTGACAGCCACT | 60 | 300 | ||
Oat2e | F GGTGTTATGAtCAGTTTGGATT | 60 | 300 | 95 |
R TTGGAGCAGTTACTGTGAGG | 58 | 300 | ||
Octl | F GAGTCACAGGGATTCTGGT | 58 | 300 | 99 |
R ACCATCCAACCGCCCTTCA | 60 | 300 | ||
Oct2 | F TGGCCTTGGAGTCTCTGG | 60 | 300 | 99 |
R TGGCGGTCCATGCTCCTTT | 60 | 300 | ||
Octnl | F GCTCTGGAATCGGGCAGAT | 60 | 300 | 100 |
| Â | R GGCCAGTGAGGGCGTTTG | 60 | 300 | |
Octn2 | F CACCGCCTCACTGGCCAACC | 60 | 300 | 112 |
R ATCTCTTCCTGGAAAGCTT | 60 | 300 | ||
Oct6 | F GCTGTAGGGAGTGGTAGTAT | 60 | 300 | 96 |
R CGATGAGCTGCGGCAGATA | 60 | 900 | ||
Orctl3 | F ACCTCGTGTCCCAGTTCATT | 60 | 300 | 100 |
R TGCATATGATCTCTGGGCGA | 60 | 300 | ||
Orctl4 | F TCACCGCCTTCCACATGTT | 59 | 300 | 96 |
R TTGGAGCCTGTCGGAGGAT | 59 | 300 | ||
Eflα | F CCTGGGAGTGAAACAGCTGATC | 60 | 300 | 96 |
R GCTGACTTCCTTGGTGATTTCC | 60 | 300 |