Fig. 7

Confirmation of microarray results by quantitative real-time PCR. The chosen genes identified by microarray analysis as differentially expressed in palisade parenchyma + upper epidermis (EPP) and spongy parenchyma + lower epidermis (ESP) from leaves of 17-day-old AtHMA4-expressing plants (line 4) and in the wild-type (WT) grown in the presence of 5 μM Zn for one week. Additionally, the expression of LeChln was analyzed. Normalized fold gene expression for a, e metal transporters and uptake facilitators as well as nicotianamine biosynthesis genes; b, f transcription factors; c, g cell wall modification; d, h ethylene pathway genes. Values correspond to means ± SD (n = 3); those significantly different from the WT (Student’s t-test) are indicated by an asterisk (P ≤ 0.05)