Fig. 1

Globin depletion of dolphin blood RNA. a By real-time PCR, HBA was observed to be significantly depleted by nearly 300-fold (t-test, p < 0.05, n = 5), with little change in other genes (t-test, p > 0.05, n = 5). b Large reductions in HBA and HBB were observed by RNA-seq analysis in the absence of expression decreases in other genes (Cuffdiff, FDR < 0.05, n = 5). Due to extremely high expression values, statistical analyses were not performed on HBA and HBB. c Globin depletion of blood RNA resulted in a 10 % increase of identified genes. Statistical significance is denoted by an asterisk