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Fig. 1 | BMC Genomics

Fig. 1

From: Biochemical and cellular characterization of transcription factors binding to the hyperconserved core promoter-associated M4 motif

Fig. 1

EMSA assay to uncover lymphocyte-specific transcription factors interacting with M4 motif bearing DNA. Radioactive γ-ATP labeled M4 oligo was incubated either with Raji burkitt’s lymphoma nuclear extracts (lane 2, 3, 4, 5, 7, 8, 9), or Hela nuclear extract (lane 10). EMSA complexes formed in assays using Raji burkitt’s lymphoma nuclear extract (lane 2, 3, 4, 5) are virtually absent in Hela nuclear extract (black arrow bar). Those band shifts were significantly faded away when an increasing amount of cold M4 motif was added (lane 2,3,4,5) but still preserved (lane 7, 8, 9) if the cold M4 motif containing oligo was replaced by an M4 scrambled counterpart. Lanes 1, 6 are control assays using M4 oligo in the absence of nuclear extracts

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BMC Genomics

ISSN: 1471-2164

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