Fig. 10

THAP11 knockdown leads to weak but significant changes in selected M4 bearing gene expression. a left panel shows fold reduction of THAP11 mRNA upon shRNA-THAP11 knockdown, right panel shows Western blot analysis of THAP11 protein from whole cell extract (WCE) of Molt4 THAP11 knockdown (shTHAP11) or "Mock" Molt4 cells (control); the amount of DNA polymerase delta cofactor PCNA was visualized by its specific antibody and served as SDS-PAGE “loading control”. b Cytosol and nuclear extract of Molt4 THAP11 knockdown (shTHAP11) or "Mock" Molt4 cells was subjected to immunoblot analysis to investigate the reduction of THAP11 protein level c Fold change of selected M4-gene expression levels upon THAP11 knockdown. Violet bar: M4-gene mRNA levels of control cells (mock Molt4) is normalized to one. Purple bar: fold change of M4-gene mRNA levels of Molt4 THAP11 knockdown cells extracted right after establishing the shTHAP11 clone (biological replicate 1). Yellow bar: fold change of M4-gene mRNA levels measured from biological replicate 2 (mRNA preparation 2 weeks after biological replicate 1) of Molt4 THAP11 knockdown cells