Fig. 2

SILAC-based DNA protein interaction sreen reveals M4 binding candidates. a Distribution of SILAC ratio against quantified proteins identified in tandem M4 DNA affinity chromatography experiments using Raji burkitt’s lymphoma nuclear extracts: The X-axis present the random code of quantified proteins originating from the mass spectrometric screening of M4 DNA affinity chromatography experiments; the (Y axis) present SILAC ratios of individually quantified proteins; b Mass spectra of selected M4 interacting partners’ derived peptides: mass spectra of individual peptides comprising both light isotope (white circle) and heavy peaks (black circle). Those derived from specific M4 binders were specified by higher intensity of heavy isotope encoded peaks. In contrast, the ones descending from unspecific binders are presented by equal intensity of both light and heavy isotope encoded peaks (in this case PARP-1)