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Table 1 Strains used in RNA sequencing experiments and respective growth conditions

From: The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets

Condition name Strain Conditionsa Concentration (mg L−1) pH MICb (μg mL−1) Reference
CaCl2 MgCl2 FeCl2
Non-induced Kp13 Original strain, no polymyxin B supplementation 25.0 12.5 0.0 7.0 32 [8]
PB Kp13PolB LB supplemented with 4 μg mL−1 of polymyxin B 25.0 12.5 0.0 7.0 64 This study
PB + high calcium Kp13Ca LB supplemented with 4 μg mL−1 of polymyxin B plus high [Ca2+] 75.0 12.5 0.0 7.0 64 This study
PB + no magnesium Kp13Mg LB supplemented with 4 μg mL−1 of polymyxin B without Mg2+ 25.0 0.0 0.0 7.0 128 This study
PB + high iron Kp13Fe LB supplemented with 4 μg mL−1 of polymyxin B plus high [Fe2+] 25.0 12.5 75.0 7.0 64 This study
PB + low pH Kp13pH LB supplemented with 4 μg mL−1 of polymyxin B in acid pH 25.0 12.5 0.0 5.8 64 This study
  1. aThe concentration of polymyxin corresponded to the concentration used during the extraction of RNA for RNAseq. All experiments were performed in duplicate. b,Minimum inhibitory concentration of polymyxin B in the respective strain