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Table 7 Summary of the sequencing methods, organisms, and mRNA extraction sources

From: Comparative transcriptomics enlarges the toolkit of known developmental genes in mollusks

Organism Class mRNA source Sequencing
Gymnomenia pellucida Neomeniomorpha 1/5 total RNA from developmental stages (i.e. freshly hatched larvae until metamorphosis) – 4/5 mRNA from adults. Illumina
Wirenia argentea Neomeniomorpha 1/7 total RNA from developmental stages (i.e. freshly hatched larvae until metamorphosis) – 6/7 mRNA from adults. Illumina
Scutopus ventrolineatus Chaetodermomorpha Total RNA from 2 adult individuals Illumina
Acanthochitona crinita Polyplacophora Early cleavage stages – gastrula – early, midstage, and late trochophore larvae – metamorphic competent and settled (post metamorphic) individuals Illumina
Idiosepius notoides Cephalopoda Central nervous system of 7 adult individuals Illumina
Idiosepius notoides Cephalopoda 2/3 total RNA from mixed developmental stages (i.e. stages collected after egg laying until the hatching stage) – 1/3 total RNA from adult central nervous system (brain), arm, and gonads tissue) 454
Lottia cf. kogamogai Gastropoda 2/3 total RNA from mixed developmental stages (i.e. trochophore – veliger – pediveliger – metamorphic competent – first juvenile stages) – 1/3 total RNA from adult foot, and central nervous system (CNS) 454
Nucula tumidula Bivalvia Early cleavage stages – gastrula – early, midstage, and late pericalymma larvae – metamorphic competent and settled (post metamorphic) individuals Illumina
Antalis entalis Scaphopoda Early cleavage stages – gastrula – early, midstage, and late trochophore larvae – metamorphic competent and settled (post metamorphic) individuals Illumina