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Fig. 7 | BMC Genomics

Fig. 7

From: Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice

Fig. 7

Expression analysis of 37 SiATG genes in response to nitrogen deficiency and darkness treatment using qRT-PCR. a 3-week old foxtail millet seedlings were kept in darkness for 48 h. b foxtail millet seeds were surfaced sterilized, and then seeds were germinated on MS and cultured for 14 days. On day 15, some seedlings were cultured on nitrogen-deficient liquid medium for 1 h or 24 h. The relative expression levels of each ATG gene in seedlings grown under darkness and nitrogen starvation were normalized to the Actin mRNA level (AF288226.1). The expression level of each ATG gene was calculated and compared with that in seedlings under normal growth conditions. ND: N deficient

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