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Table 1 Standard Erythroid Media (SEM) components. Erythroid progenitors were cultured in two Standard Erythroid Medium modifications SEM-F or SEM-i as indicated in methods

From: Distinct gene expression program dynamics during erythropoiesis from human induced pluripotent stem cells compared with adult and cord blood progenitors

Component added to IMDM (Biochrom) Source SEM-F SEM-i
   Phase I
(Days 1-8)
Phase II
(Days 8-11)
Phase III
(Day 11 onward)
Phase I
(Days 1-2)
Phase II
(Days 2-8)
Phase III
(Days 8-14)
Phase IV
(Day 14 onward)
Fetal Bovine Serum (FBS) Sigma 2 % 2 % 2 % 3 % 3 % 3 % 3 %
Penicillin Sigma 100 U/ml 100 U/ml 100 U/ml 100 U/ml 100 U/ml 100 U/ml 100 U/ml
Streptomycin Sigma 100 μg/ml 100 μg/ml 100 μg/ml 100 μg/ml 100 μg/ml 100 μg/ml 100 μg/ml
Glutamine Sigma 4E-3M 4E-3M 4E-3M 4E-3M 4E-3M 4E-3M 4E-3M
Human AB serum Sigma 3 % 3 % 3 % 2 % 2 % 2 % 2 %
Folic Acid Sigma 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml
Inositol Sigma 40 μg/ml 40 μg/ml 40 μg/ml 40 μg/ml 40 μg/ml 40 μg/ml 40 μg/ml
Holotransferrin Sigma 200 μg/ml 1 mg/ml 1 mg/ml 120 μg/ml 120 μg/ml 1 mg/ml 1 mg/ml
Human Recombinant Erythropoietin (Epo) Janssen 3 U/ml 3 U/ml 3 U/ml 3 U/ml 3 U/ml 3 U/ml 3 U/ml
Insulin Sigma 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml 10 μg/ml
Monothioglycerol Sigma 1.6E-4M 1.6E-4M 1.6E-4M 1.6E-4M 1.6E-4M 1.6E-4M 1.6E-4M
Cholesterol-Rich Lipids Sigma 40 μg/ml 40 μg/ml 40 μg/ml 40μg/ml 40μg/ml 40μg/ml 40 μg/ml
Heparin Sigma 3 U/ml 3 U/ml 3 U/ml 3 U/ml 3U/ml 3U/ml 3 U/ml
Human Stem Cell Factor (SCF) R&D Systems 100 ng/ml 100 ng/ml 0 100 ng/ml 100 ng/ml 100 ng/ml 0
Dexamethasone Sigma 1E-6M 0 0 1E-6M 1E-6M 1E-6M 1E-6M
IL3 R&D Systems 1 ng/ml 0 0 5ng/ml 5ng/ml 0 0
FLT3 Ligand R&D Systems 0 0 0 20 ng/ml 20 ng/ml 20 ng/ml 0
IL6 R&D Systems 0 0 0 10 ng/ml 0 0 0
Thrombopoietin R&D Systems 0 0 0 20 ng/ml 0 0 0
  1. IMDM Iscove’s Modified Dulbecco’s Medium; IL3 interleukin-3; BSA bovine serum albumin; FLT3 Fms-like tyrosine kinase 3; IL-6 interleukin-6