Component added to IMDM (Biochrom) | Source | SEM-F | SEM-i | |||||
---|---|---|---|---|---|---|---|---|
 |  | Phase I (Days 1-8) | Phase II (Days 8-11) | Phase III (Day 11 onward) | Phase I (Days 1-2) | Phase II (Days 2-8) | Phase III (Days 8-14) | Phase IV (Day 14 onward) |
Fetal Bovine Serum (FBS) | Sigma | 2 % | 2 % | 2 % | 3 % | 3 % | 3 % | 3 % |
Penicillin | Sigma | 100 U/ml | 100 U/ml | 100 U/ml | 100 U/ml | 100 U/ml | 100 U/ml | 100 U/ml |
Streptomycin | Sigma | 100 μg/ml | 100 μg/ml | 100 μg/ml | 100 μg/ml | 100 μg/ml | 100 μg/ml | 100 μg/ml |
Glutamine | Sigma | 4E-3M | 4E-3M | 4E-3M | 4E-3M | 4E-3M | 4E-3M | 4E-3M |
Human AB serum | Sigma | 3 % | 3 % | 3 % | 2 % | 2 % | 2 % | 2 % |
Folic Acid | Sigma | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml |
Inositol | Sigma | 40 μg/ml | 40 μg/ml | 40 μg/ml | 40 μg/ml | 40 μg/ml | 40 μg/ml | 40 μg/ml |
Holotransferrin | Sigma | 200 μg/ml | 1 mg/ml | 1 mg/ml | 120 μg/ml | 120 μg/ml | 1 mg/ml | 1 mg/ml |
Human Recombinant Erythropoietin (Epo) | Janssen | 3 U/ml | 3 U/ml | 3 U/ml | 3 U/ml | 3 U/ml | 3 U/ml | 3 U/ml |
Insulin | Sigma | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml | 10 μg/ml |
Monothioglycerol | Sigma | 1.6E-4M | 1.6E-4M | 1.6E-4M | 1.6E-4M | 1.6E-4M | 1.6E-4M | 1.6E-4M |
Cholesterol-Rich Lipids | Sigma | 40 μg/ml | 40 μg/ml | 40 μg/ml | 40μg/ml | 40μg/ml | 40μg/ml | 40 μg/ml |
Heparin | Sigma | 3 U/ml | 3 U/ml | 3 U/ml | 3 U/ml | 3U/ml | 3U/ml | 3 U/ml |
Human Stem Cell Factor (SCF) | R&D Systems | 100 ng/ml | 100 ng/ml | 0 | 100 ng/ml | 100 ng/ml | 100 ng/ml | 0 |
Dexamethasone | Sigma | 1E-6M | 0 | 0 | 1E-6M | 1E-6M | 1E-6M | 1E-6M |
IL3 | R&D Systems | 1 ng/ml | 0 | 0 | 5ng/ml | 5ng/ml | 0 | 0 |
FLT3 Ligand | R&D Systems | 0 | 0 | 0 | 20 ng/ml | 20 ng/ml | 20 ng/ml | 0 |
IL6 | R&D Systems | 0 | 0 | 0 | 10 ng/ml | 0 | 0 | 0 |
Thrombopoietin | R&D Systems | 0 | 0 | 0 | 20 ng/ml | 0 | 0 | 0 |