Fig. 1
Overall design of experiment. Inner Mongolia Cashmere goat fetal fibroblasts (GFbs) were maintained as monolayer cultures in Dulbecco modified Eagle’s medium (DMEM)/F12, supplemented with 10 % fetal bovine serum and penicillin/streptomycin. Untreated GFbs and GFbs that were treated with 50 nM CCI-779 (temsirolimus) for 12 h were cultured in petri dishes (diameter = 100 mm), and 5.8 × 106 cells were collected for each sample. The cells processing were repeated 3 times and to isolate total RNA separately. Then, the 3 isolated total RNAs were mixed for RNA-seq