Fig. 10

Forty differentially expressed genes (DEGs) in RNA-seq were validated by qPCR and show a high concordance. Comparison between RNA-Seq and qPCR results. X-axis shows genes validated in this study, among which some genes have different gene name and symbol, including GST (LOC102179473, LOC102189581, LOC102188913), mmsB (PSIP1), bdh (BDH2), CYP 1B1 (LOC102184252), PIK3C (PIK3CD), ATGL (LIPG), FNTB (CHURC1), udk (UCK1), ndk (LOC102176263), Cyt b5R (LOC102173645), OGT (TTC5), ACH1 (STARD10), VEGF (LOC102177848), RPB1 (ODF3L2); Y-axis shows log2ratio of expression of CCI-779 treatment versus control group. Positive value means upregulation, and negative value means downregulation. The red column represents qPCR results, and the blue column represents the RNA-Seq results. The results of the qPCR analysis for 40 genes with a significant difference and important function in amino acid, lipid, carbohydrate, and single nucleotide metabolism, RNA polymerases, transcription factors and mTOR signaling show high concordance with RNA-Seq findings