Fig. 6

SOX10 regulates the expression of genes that inhibit glial cell differentiation. a Eight genomic segments at the rat Notch1, Hmga2, Hes1, Mycn, Id4, and Id2 loci were cloned upstream of a luciferase reporter gene in both the forward and reverse orientations and tested for luciferase activity in rat Schwann (S16) cells. Luciferase data are expressed relative to a control vector with no genomic insert (‘Empty’). Error bars represent standard deviations. b The conserved SOX10 consensus sequence(s) were deleted in each of the seven regions that were active in a (see text for details). Luciferase reporter gene constructs containing the wild-type sequence (WT) or the sequence lacking the SOX10 consensus sequence(s) (ΔSOX10) were transfected into S16 cells and luciferase assays performed. Luciferase activities are expressed relative to the wild-type expression constructs and error bars represent standard deviations. c Rat Schwann (S16) cells were treated with a control siRNA or a siRNA targeted against Sox10. Quantitative RT-PCR was used to measure expression levels of each indicated gene. Asterisks indicate a p-value smaller than 0.001 and error bars indicate standard deviations. d RNA was purified from three independent rat sciatic nerves at the P1, P15, and adult timepoints. Quantitative RT-PCR was used to measure expression levels of each indicated gene with values expressed relative to expression levels at P1. Asterisks indicate a p-value smaller than 0.005 and error bars indicate standard deviations