Fig. 4

Differential unigenes expression in response to viral infections. Venn diagrams present the number of discrete and shared differentially expressed (both up and down regulated; 0.05 FDR-adjusted p < 0.05) unigenes identified by either DESeq2 (left), edgeR (right), or those common (central) to both analyses. Analyses were conducted using a reference transcriptome containing only genes with >0.5 cpm present in at least four libraries. Treatment-specific comparisons are presented as related to (a) PRV infection, (b) IHNV exposure and/or infection, or (c) those specific to PRV and IHNV co-infection. In each instance, collection relative to dpc PRV, challenge relative to PRV and/or IHNV, and IHNV kidney infection status (+/−) following exposure are indicated along with the experimental condition group number (see Table 1)