De novo assembly and analysis of changes in the protein-coding transcriptome of the freshwater shrimp Paratya australiensis (Decapoda: Atyidae) in response to acid sulfate drainage water

Table 2 Gene Ontology annotation statistics for deduced proteins corresponding to all transcripts above a minimum abundance threshold and differentially expressed subsets of these transcripts

	All transcripts with read coverage of > 100 in any library	Downregulated >2-fold in either 50 % or 100 % ASDW	Upregulated >2-fold in either 50 % or 100 % ASDW
Total number of deduced protein sequences	14,607	85	116
Sequences with annotations^a	6293	17	26
Sequences that could not be annotated^a	8314 (56.9 %)	68 (80 %)	90 (77.6 %)
Sequences with any functional inference^b	9544	39	49
Sequences with no GO annotation	5063 (34.7 %)	46 (54.1 %)	67 (57.8 %)

Analysis was limited to transcripts with abundance in terms of raw read coverage of 100 reads per transcript from any single library
^aAccording to BLAST2GO analysis, based on BLAST results only
^bAccording to BLAST2GO analysis, based on BLAST results, domain homology searches using InterProScan, and ANNEX results

ISSN: 1471-2164