Fig. 1

Cold shock treatment in zebrafish larvae. a Relative expression levels of cirbp gene in zebrafish larvae at 96 h post fertilization (hpf) were determined by q PCR at different time point post transfer from 28.5 to 18 °C. * and ** indicates p ≤ 0.05 and 0.01, respectively, comparing to the control at 0 h post transfer. b Scheme of cold exposure. Embryos were incubated at 28.5 °C until 96 hpf then transferred to 18 or 28.5 °C for an additional 4 h. Larvae before (control) and after treatments (normal and cold shock) were then collected for RNA-seq and miRNA-seq analyses as described in the Methods