Fig. 6
From: Systematic identification of Ctr9 regulome in ERα-positive breast cancer
PAFc-independent role of Ctr9 in RNA polymerase II chromatin association at GREB1 and IGFBP4 genes assessed by ChIP-qPCR. a ChIP-qPCR analyses of the occupancy of RNAPII on the TSS or TR of GREB1 and IGFBP4 genes in MCF7 cells stably expressing control shRNA or shRNAs targeting Ctr9, Cdc73, Ski8, and PAF1 treated with DMSO or 10 nM E2 for 45 min. b ChIP-qPCR analyses of the occupancy of IgG control on the TSS or TR of GREB1 and IGFBP4 genes in MCF7 cells stably expressing control shRNA or shRNAs targeting Ctr9, Cdc73, Ski8, and PAF1 treated with DMSO or 10 nM E2 for 45 min. ChIP-qPCR data were normalized to input DNA qPCR data and expressed as “percent of input”; data are represented as mean ± SD. n = 3