Table 3 SNP and InDel distribution in amino acid biosynthetic pathway

ATCC21300

lysine

ATCC13032

ppc: upstream -1 A deletion;

……

ppc: phosphoenolpyruvate carboxylase

B253

lysine

B1

lysC: p.Leu329_Gln330del (inframe deletion), p.Gly359Asp;

hom: p.Gln399* stop gained

lysC: Aspartokinase

hom: Homoserine dehydrogenase

ATCC21493

arginine

ATCC14067

KIQ_011285: p.Gly159Asp;

KIQ_013990: p.Arg390Cys;

KIQ_009960: Ala701Thr p.Ala378Thr

KIQ_011285: arginine repressor

KIQ_013990: glutamate_dehydrogenase

odhA(KIQ_009960): 2-oxoglutarate dehydrogenase E1/E2 component

SYPS-062

serine

ATCC14067

KIQ_000725: p.Leu103Phe;

KIQ_012535: p.Glu251Lys, p.Arg422Gln;

KIQ_009375: p.Asp394Asn;

KIQ_009610: upstream-9 C->T

KIQ_000725: serine acetyltransferase

KIQ_012535: serine dehydratase

KIQ_009375: serine_hydroxymethyltransferase

KIQ_009610: phosphoglycerate mutase

KIQ_014800: pyruvate dehydrogenase E1

SYPS-062-33a

serine

ATCC14067

KIQ_000725: p.Leu103Phe;

KIQ_012535: p.Glu251Lys, p.Arg422Gln;

KIQ_009375: p.Asp394Asn;

KIQ_009610: upstream-9 C->T;

KIQ_014800: p.His594Tyr

ATCC15168

isoleucine

ATCC14067

KIQ_005265: p.Ser248Phe;

KIQ_012240: p.Gly186Arg

KIQ_005265:2-isopropylmalate synthase;

KIQ_012240: phosphoenolpyruvate carboxylase

MT

arginine

AS1.542

argR: p.Gln37*stop gained;

odhA: p.Ala170Thr;

argC; p.Gly134Glu

argR Arginine repressor

argC: N-acetyl-gamma-glutamyl-phosphate reductase

argG: Argininosuccinate synthase

argF: Ornithine carbamoyltransferase

odhA: 2-oxoglutarate dehydrogenase E1/E2 component

SYPA5-5

arginine

AS1.542

argR: p.Gln37* stop gained;

odhA: p.Ala170Thr;

argC: p.Gly134Glu, p.Asp123Asn;

argG: p.Ile219Thr;

argF: p.Ala191fs

SCgG1

glutamate

T6-13

dapA: p.Glu293Lys;

ppc: p.Ala433Thr

dapA: 4-hydroxy-tetrahydrodipicolinate synthase

ppc: phosphoenolpyruvate carboxylase

ykuT(yggB): putative MscS family protein YkuT

aceF: Dihydrolipoyllysine-residue acetyltransferasecomponent of pyruvate dehydrogenase complex

SCgG2

glutamate

T6-13

dapA: p.Glu293Lys;

ppc: p.Ala433Thr

Z188

glutamate

T6-13

dapA: p.Glu293Lys;

ppc: p.Ala433Thr;

ykuT: p.Glu350Lys

S9114

glutamate

T6-13

dapA: p.Glu293Lys;

ppc: p.Ala433Thr;

ykuT: p.Glu350Lys;

aceF: p.Glu216Asp, p.Glu344Gln, p.Lys365 Pro369del