Fig. 3

Orthologous and paralogous members of the Ceacam family in X. tropicalis and X. laevis. Phylogenetic trees were constructed based on mature N domain amino acid sequences (signal peptides excluded) from X. tropicalis and X. laevis group 1 (a) and group 2 Ceacams (b) using the Maximum Likelihood method (MEGA6 software). The tree with the highest log likelihood is shown. The percentage of trees in which the protein sequences clustered together is shown next to the branches. For Ceacams predicted to contain more than one N domain, only the most N-terminal N1 domains were included in the analysis. Ceacams whose N domains exhibit the highest degree of identity within the same species (paralogs) are boxed with red and blue lines for X. tropicalis and X. laevis, respectively. Pairs consisting of most closely related proteins from different species (sometimes including recently generated paralogs) are highlighted in gray (orthologs). The names of ITIM-containing members are highlighted in red, proteins with ITAM and ITAM-like motifs are marked with green and blue background, respectively. P, pseudogene; Xla, X. laevis; Xtr, X. tropicalis