Genomic determination of minimum multi-locus sequence typing schemas to represent the genomic phylogeny of Mycoplasma hominis

Table 3 PCR Primers: Proposed PCR primers to be used to amplify the 7 MLST loci

Gene	Forward	Reverse
MHO_4840^a GENE-20	AATGAACCTATTTATTTTTTGGGTG	AATTTTGAATAAACTGGTATTTCTTTG
MHO_0720^a GENE-58	CTGCTGCAGCACTTATTGC	GAACGTGATAAAGGAACTACTCA and CGCGATAAAGGAACTACTCA ^b
secD GENE-94	GGATGGGATAGTTTTGTGC	ATCTATTTTGATTTGTTGAACTACC
oppA GENE-138	GTTACAGTTAAGAGCTTTGATG	ATTGATATAGATCGGTTGGTTC
argS GENE-183	CATGGCGGAGATATGATAGA	TTGCATCATTTCCAACTTCTTC
hiss GENE-236	CTTTTGAGTCAAGAAATAACTACAT	TTTTTTTCATCTTCATTCAAATAAGCAA
MHO_1160^a GENE-265	TTTTAGAAGATTTTATTTGCCCACA and TTTAGAAGATTTTATTTGCCCGC ^b	TAAAGTCGCCATTAGCCTG
tyrS GENE-428	CTTGCTTCAAGGTTGAGATTTTA	CTTTTGGTTTTATTTTCATTGTGTTG
dnaG GENE-519	CTTCCCACTTCATCCTATTTC and ACTTTCCACTTCATCCTATTTCAA ^b	GATTGGCCTGTTTCTTTCATTC

^a gene encoding a hypothetical protein. Gene name relates to annotation in ATCC 23114
^b due to SNP in the primer footprint, equal volumes of 2 primers are required to ensure amplification of the target.

ISSN: 1471-2164