Fig. 3

Binding of transcription factors to visfatin promoter variants as measured in-vitro by EMSA. a EMSA for c.-3187G > A SNP only, b EMSA for c.-1537C > T SNP only, c Assessment of binding between visfatin SNPs and nuclear protein, the EMSA band density are indicated in shaded bars and the standard deviation (SD) between 2 EMSA experiments is represented by the error bars. (1): Wild-type sequence without nuclear protein, (2): Wild-type sequence with nuclear protein, (3): Wild-type sequence with nuclear protein and unlabeled probe, (4): Variant sequence without nuclear protein, (5): Variant sequence with nuclear protein, (6): Variant sequence with nuclear protein and unlabeled probe