Fig. 4
From: Knockdown of POLA2 increases gemcitabine resistance in lung cancer cells
Effects of POLA2 gene silencing. a Protein expression after knockdown of POLA2 by siRNA. Comparison of POLA2 protein expression in H1299 cell line by Western blotting after 48 and 72 h of siRNA transfection. Internal control for equal loading: glyceraldeyde phosphate dehydrogenase (GAPDH). b Chemosensitivity assay by comparing IC50 values before and after knockdown of POLA2. Results indicated that the H1299 cell line became more resistant to gemcitabine treatment after siRNA knockdown of POLA2 (Knockdown), as compared to the non-transfected H1299 cell line having wild-type POLA2 (Control). Experiments were conducted in triplicates and repeated at least 3 times. Data are shown as the mean ± SD (*p <0.05)