Table 7 Effect of DNA sub-sites (3-bp) position on the zinc finger binding specificity
Experimental | Approach 3 | ||||
|---|---|---|---|---|---|
DNA Target sequence 5’ – 3’ | DNA sub-site position w.r.t to F1, F2, F3 | Corresponding Helix | IHBE score for experimental DNA target and corresponding Helix | ||
GGG | Jamieson et al. 1996 [32] | GGG | 1 | RER | 0.0 |
Segal et al. 1999 [45] | GGG | 2 | RHR | 39.3 | |
Van et al. 2004 [46] | GGG | 3 | RHR | 50.63 | |
GCG | Hurt et al. 2003 [44] | GCG | 1 | RER | 30.9 |
Van et al. 2004 [46] | GCAGCGGAG | 2 | RER | 21.64 | |
Kim et al. 2010 [43] | GCGGGGGCG | 3 | RER | 28.6 | |
GTG | Jamieson et al. 1996 (32) | GTG | 1 | RER | 1.35 |
Hurt et al. 2003 [44] | GCGGTGGCG | 2 | RER | 19.59 | |
Van et al. 2004 [46] | GTGGACGAA | 3 | RAR | 5.5 | |
GAC | Rebar et al. 2002 [39] | GGGGGTGAC | 1 | DNR | 2.04 |
Holmes et al. 2005 [40] | GTGGACGAA | 2 | DNR | 4.033 | |
Bae et al. 2003 [41] | GAC | 3 | CNR | 7.23 | |