Fig. 4

Comparison between results obtained with Corset- or RSEM-estimated transcript abundances, and RT-qPCR. a Venn diagrams depicting the number of up- and down-regulated contigs in mirids feeding on JA-silenced Nicotiana attenuata plants identified by RNAseq analysis. Among the down-regulated genes, a list of differentially-expressed (DE) contigs annotated as detoxification enzymes or serine proteases (ser. prot) is shown; b) and c) Scatter plots showing the correlation between log2 fold-change (log2FC) estimated by RNAseq or by RT-qPCR for putatively unique transcripts (PUTs) likely involved in N. attenuata metabolite detoxification. Asterisks indicate contigs that have been identified as DE by RNAseq analysis using both Corset- or RSEM-estimated transcript abundances