Exposure of bovine oocytes and embryos to elevated non-esterified fatty acid concentrations: integration of epigenetic and transcriptomic signatures in resultant blastocysts

Table 1 Primers and annealing temperatures used for pyrosequencing

	Forward primer	Reverse primer	Sequencing primer	Annealing temperature
COL6A3	AGGGAAGGTTGTTTAGAGGA	ACCTAAAAATACTAATAACTAATCCATCA	GGTTTTATATATAAGTAAAAGAA	57 °C
APAF1	TTTGGATGTTTTGTTAGATTGTAGTT	AACTTACCACTACTACCCTCTAC	ATTGGGTTTTGTGTG	54 °C
RFC4	TATTTTAAAGGAGATTAGTTTTGGGTGTT	CTCAATTCAATCATAACTCAATCCTATA	ATTGATGTTGAGGTTGAAA	60 °C
PLSCR3	TGGTTTTGTTGTTTGGTAGTTAAT	CTCATATAACCAAACCCTTAATCTATC	AGTTTTTTTATTTTAGTAGTTGG	54 °C
ELOVL1	GTTTTGTAGAGTTGGTGAGTGTA	ATTTACTATCTAACCAAATCACTTCAC	AGTTGGTGAGTGTATG	59 °C
TBKBP1	AGAGATGAGAAGGTTAGAGATTTAAGTAT	AACCCTACCACATCTACATCCTCATA	GTTTTAAATTAGTTGGGAGATA	58 °C
ERCC1	TTTTTAGTTTGGATATATAGATATGGGA	ACTCAACCCCACTCATCT	GGATATATAGATATGGGAAATATT	52 °C
CELF2	AGGAGAGAGGTGGTAATAATAAAGTTATA	AACCAAAACCCTTTTCTCCA	GTTGGTGATGAATTTGTAG	53 °C
ZFAND6	TGGGGAGGAGTATATGTGTATT	CTTATTCTACCATTACTACTATTCTATCTT	ATATGTGTATTTTTTTTTATTATAT	55 °C
PREX1	GGTTGGGTATTTATGTTTAGAAATTAAGT	ACCTTCTCTATAATATTTATTCCTCTACC	ATTAAGTGAGAAGGTATTTG	56 °C
APPL2	GTAGGTTTTATGGGGAAATTATGTAT	TTCCATACCCTACCTATAAAAAATCTTC	GTTTTTTTTTTTTATTGGGTAGA	55 °C
GCLC	GTTAAATGGATGAGGGAGTTTAT	ATTTTACTTCCACTATACCTAACTTT	TGAATTATAGTTTTTTTTTAAAGTG	52 °C

ISSN: 1471-2164