Table 2 Characteristics of four long-range mtDNA PCR primers used by Li et al [27] and four proposed high fidelity long-range primers
Primers set | Primer | Sequence | Number of similar sequences in nDNA | Primer length/LSS starting from primers position | Number of positions in mtDNA with less value of LSS | Percent of positions in mtDNA with less value of LSS |
|---|---|---|---|---|---|---|
Long-range mtDNA PCR primers used by Li et al [25] | 10 F (forward_10kb) | CCGTGCAAAGGTAGCATAATC | 8 | 21/84 | 11,965 | 72.21% |
10R (reverse_10kb) | TTACTTTTATTTGGAGTTGCACCA | 16 | 24/52 | 9,125 | 55.07% | |
8 F (forward_8kb) | GGCTTTCTCAACTTTTAAAGGATA | 10 | 24/34 | 6,504 | 39.25% | |
8R (reverse_8kb) | TGTCCTGATCCAACATCGAG | 14 | 20/72 | 11,089 | 66.93% | |
Proposed long-range mtDNA PCR primers | AF (forward_10kb) | TACTACCAGACAACCTTAGCCA | 0 | 22/18 | 218 | 1.32% |
AR (reverse_10kb) | GAGTCATAAGTGGAGTCCGTAA | 0 | 22/18 | 217 | 1.31% | |
BF (forward_8kb) | CACCATTTCCGACGGCATCTAC | 0 | 22/16 | 1 | 0.01% | |
BR (reverse_8kb) | TGCGCCAGGTTTCAATTTCTAT | 0 | 22/18 | 217 | 1.31% |