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Table 5 Real-time PCR confirmation of the microarray results

From: Genome-wide expression profiling in muscle and subcutaneous fat of lambs in response to the intake of concentrate supplemented with vitamin E

 

Microarray

qPCR

Gene

VE vs CON

VE vs CON

L.Thoracis

  CISH

−2.05***

−3.07**

  ABCC4

−1.22***

−1.07

  ACAT1

−1.25***

−1.42

  IGF1R

−1.17**

−1.03

Subcutaneous fat

  LDLR

2.66****

2.25

  METLL1

1.53****

1.28

  SOD3

−2.78****

−3.51**

  SQLE

2.70****

2.24

  SREBF1

1.76****

1.73

  HAX1

1.44****

1.21

  HMGB2

−1.53****

−2.24

  HSPB8

1.87****

1.86

  IER3

−2.06****

−2.54*

  1. Gene expression changes in L. Thoracis muscle (CISH, ABCC4, ACAT1 and IGF1R) and subcutaneous fat (LDLR, SOD3, SQLE, SREBF1, HAX1, METLL1, HMGB2, HSPB8 and IER3) by the fold change (FC) obtained with microarray and qPCR data and their significance (P < 0.1*; P < 0.05**; P < 0.01***; P < 0.001****)
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BMC Genomics

ISSN: 1471-2164

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