Detecting very low allele fraction variants using targeted DNA sequencing and a novel molecular barcode-aware variant caller

Table 3 Estimated optimal cutoffs based on various false positive rates (FPR) at 20, 50, and 100 per megabase and barcode depths from 500 to 3,500. The prediction equations at each FPR is also given

FPR (Mb⁻¹)	Prediction equation	500	1000	1500	2000	2500	3000	3500
20	y=14+0.012x	20	26	32	38	44	50	56
50	y=15+0.0092x	20	25	29	34	38	43	48
100	y=13+0.0088x	18	22	27	31	35	40	44

ISSN: 1471-2164