Fig. 2

New annotation for the Ae. aegypti genome. a Vectorbase annotation of the PIT proteome that maps to Ae. aegypti (hypoth. denotes ‘hypothetical’ protein). b Previously published transcriptomic and proteomic evidence for the expression of our PIT-identified proteins that are either annotated or listed as ‘hypothetical’ or ‘conserved hypothetical’ (hypothetical) in Vectorbase (source data specified in Additional file 6). c Result of Vectorbase BLAST alignment against the Ae. aegypti genome of all 215 PIT transcripts initially marked as having homology to proteins from Cx. quinquefasciatus or D. melanogaster, but not Ae. aegypti (‘non-Aedes’ insect hits). d Mean transcript length of non-Aedes insect PIT hits that do (mapped) or do not (no match) map to the Ae. aegypti genome. Error bars represent standard error of the mean; * P < 0.001. e Examples of non-Aedes insect PIT transcripts mapping precisely (i), or as extensions or transcript variants (ii) of Ae. aegypti genes already annotated in Vectorbase (Trinity IDs 4627 and 1476 respectively). f Examples of non-Aedes insect PIT transcripts providing new genomic annotation as potentially novel ORFs (i to iii), or extensions or transcript variants of known ORFs (ii and iii) (Trinity IDs 3521, 1935 and 1124 respectively). (d and e) Contigs not shown in entirety; illustrations are modified Vectorbase BLAST alignments of representative PIT transcripts (images stylistically edited for clarity); <> indicates annotated transcript orientation; filled boxes represent regions of alignment to genome (exons)