Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library

Table 2 Growth and alginate production of mutants using medium supplements or complementing transposons^a

Inactivated gene	Supplement/comple-menting gene (s)^b	PIA		0.5xDEF4 Glycerol		0.5xDEF4 Fructose
Inactivated gene	Supplement/comple-menting gene (s)^b	Growth^c	Alginate	Growth^c	Alginate	Growth	Alginate
wt		100	100	100	100	100	100
trpF		65	0	145	0	291	38
	tryptophane	70	139	78	60	130	45
	trpF	88	121	88	94	153	106
trpD		56	0	1	0	3	0
	tryptophane	68	261	68	80	71	75
	trpD	85	142	114	23	242	38
	trpDC	89	85	81	91	152	120
purH		29	0	1	0	21	0
	Adenine, thiamine	51	188	14	33	39	21
	purH	90	100	91	88	163	97
purE		33	0	0	0	1	0
	Adenine, thiamine	56	91	17	50	43	29
	purE	57	0	1	0	1	0
purL		19	0	0	0	1	0
purL	Adenine, thiamine	52	124	12	48	38	30
ilvD		77	18	2	2	4	0
ilvD	ilvD	91	82	91	109	222	97
aceEI		33	58	6	20	13	7
aceEI	aceEI	96	42	85	96	244	123
PFLU3030		88	0	80	89	104	103
PFLU3030	PFLU3030	97	142	89	123	101	111
dsbC		108	52	111	89	149	115
dsbC	dsbC	105	127	105	117	94	92
sohB		100	9	100	50	479	−2
sohB	sohB	91	109	104	106	109	100
nagZ		53	0	139	93	115	75
nagZ	nagZ	87	145	84	120	289	79
anmK		32	0	124	98	197	99
anmK	anmK	84	118	86	135	111	128
ispA		82	0	100	97	121	115
ispA	ispA	92	212	88	117	405	77
cbrB		83	0	110	63	374	46
cbrB	cbrB	91	118	91	95	213	107
PFLU3887		91	67	98	103	105	110
PFLU3887	PFLU3887	90	33	109	103	112	103
PFLU5567		87	64	2	6	141	30
PFLU5567	PFLU5567	93	103	1	16	6	10

a: The strains were grown in deep-well plates containing the indicated media for four days before cell and alginate yield were measured. b: empty field denotes no supplement or complementing vector. c: Values are given as percentage of the control strain (SBW25 MS1 ΔalgC:: TnKB61). Actual values for the control strain were (growth [OD₆₆₀]/alginate [g/L]): PIA: 0.492/0.33, DEF4 glycerol: 0.850/1.72, DEF4 fructose: 0.308/3.08. Growth above 125% and alginate production above 110% are marked using bold types, growth and alginate production between 10 and 50% are marked using italics, and growth and alginate production below 10% are written in bold italics

ISSN: 1471-2164