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Table 3 Effect of PhoBR disruptions on P. fluorescens growth and alginate biosynthesis

From: Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library

Strain

Growth (OD600)

Alginate (g/l)

SBW25mucAHE230

2.5+/−0.24

4.3+/−0.89

SBW25mucAHE230 ΔphoR

2.7+/−0.33

3.6+/−0.36

SBW25mucAHE230 ΔphoB

1.4+/−0.12

0.0+/−0.0

SBW25mucAHE230 ΔphoR:: TnTK5

2.4+/−0.09

4.0+/−0.24

SBW25mucAHE230 ΔphoR:: TnTK7

2.1+/−0.10

4.3+/−0.66

SBW25mucAHE230 ΔphoB:: TnTK6

1.5+/−0.20

1.2+/−0.56

SBW25mucAHE230 ΔphoB:: TnTK7

2.0+/−0.13

5.5+/−0.12

  1. a: The cells were grown for 72 h in shaking flasks using DEF3 medium with 20 g/l glycerol, 1 μM phosphate and 0.5 mM m-toluate. Average values from three independent experiments are shown
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BMC Genomics

ISSN: 1471-2164

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