Strains | Description | Reference |
---|---|---|
E. coli S17-1 (λpir) | λpir (for replication of oriR6K-plasmids) recA, thi pro hsdR-M + RP4 2-Tc::Mu-Km::Tn7TpRSMR | [36] |
P. fluorescens SBW25 | Non-mucoid P. fluorescens wild type | [37] |
SBW25MS1 | Derivative of SBW25 where the Pm promoter is inserted directly upstream of algD using pMS9. | This study |
SBW25MS1 ΔalgC:: TnKB61 | Derivative of SBW25 MS1 where algC has been deleted utilizing pKB22, and a copy of algC controlled by the PmG5 promoter has been introduced on a transposon inserted into gene PFLU2944. | This study |
SBW25mucAHE230 | Alginate-producing derivative of SBW25 encoding a defect MucA and where the expression of algC is controlled by the PmG5 promoter | H. Ertesvåg, unpublished |
SBW25mucAHE230ΔphoB | Derivative of SBW25mucAHE230 where an in-frame deletion in phoB was introduced utilizing pTK10. | This study |
SBW25mucAHE230ΔphoR | Derivative of SBW25mucAHE230 where an in-frame deletion in phoR was introduced utilizing pTK9. | This study |
Plasmids | ||
pKD20 | pUT based transposon vector containing PmG5. Apr, Kmr. | [5] |
pLitmus28Tc | High copy number cloning vector. Tcr, Apr | [5] |
pMG48 | RK2-based gene replacement vector. lacZ + , Tcr, Apr | [33] |
pMC1 | RK2-based gene replacement vector for replacing the DNA sequence upstream of algD with the Pm-promoter. lacZ + , Tcr, Apr | [10] |
pKB22 | Gene replacement vector for creating an algC-deletion. lacZ + , Tcr, Apr | [5] |
pKB60 | Transposon vector. Contains the transposon TnKB60 with algC under the control of PmG5. Apr, Kmr | [5] |
pYQ1 | pUT based transposon vector containing PmG5. Amr, Kmr. | [2] |
pEM1 | Derivative of pLitmus28Tc containing part of the transposon from pKD20. Apr | [2] |
pKB61 | Derivative of pKB60 where a 1.7 kb AvrII-NcoI DNA fragment encoding Kmr and most of XylS was exchanged with a 2.5 kb AvrII-NotI DNA fragment containing tetAR. Tcr, Apr | This study |
pMS9 | Derivative of pMC1 where a 0.7 kb SbfI-NotI DNA fragment containing a gene upstream of algD was exchanged with a PCR product containing the 0.8 kb sequence directly upstream of algD. lacZ + , Tcr, Apr | This study |
pMS2 | Derivative of pLitmus28Tc where the tetAR genes were exchanged with a 3.4 kb BamHI-fragment from pKD20 containing the minitransposon and oriR6K. Kmr, Apr | This study |
pMS10 | Derivative of pMS2 where a 0.5 kb BsiWI-EcoRI-fragment containing oriR6K was deleted and the 1.9 kb NotI-PstI fragment encoding XylS was exchanged with a 0.4 kb PCR product encoding oriR6K. Kmr, Apr | This study |
pMS11 | Derivative of pKD20 where a 3.7 kb BssHII-SfiI-fragment was exchanged with a 1.5 kb BssHII-SfiI-fragment containing oriR6K from pMS10. Kmr, Apr | This study |
pTK1 | Derivative of pEM1 in which a 2.2 kb PCR-amplified NdeI-NotI DNA fragment encoding phoBR from P. fluorescens was inserted. Apr. | This study |
pTK3 | Derivative of pTK1 in which an inserted 2.2 kb PCR-amplified NcoI-PspOMI DNA fragment from P. fluorescens including the first 46 nt of phoB replaced most of the phoB gene. Apr. | This study |
pTK4 | Derivative of pTK1 from which a 0.9 kb BstEII-BsaBI DNA fragment encoding most of phoR was deleted. Apr. | This study |
pTK5 | Derivative of pKD20 in which a 1.5 kb NdeI-NotI PCR fragment from P. fluorescens containing phoR was inserted. Kmr. | This study |
pTK6 | Derivative of pKD20 in which a 1.1 kb NcoI-NotI DNA fragment from pTK4 containing phoB was inserted. Kmr. | This study |
pTK7 | Derivative of pKD20 in which a 2.2 kb NdeI-NotI PCR fragment from P. fluorescens containing phoBR was inserted. Kmr. | This study |
pTK8 | Derivative of pTK1 from which a 0.3 kb BstEII DNA fragment was deleted, creating an in-frame deletion in phoR. Apr. | This study |
pTK9 | Derivative of pMG48 in which a 3.0 kb NcoI-NotI DNA fragment from pTK8 was inserted, containing a deletion in phoR. Apr, Tcr. | This study |
pTK10 | Derivative of pMG48 in which a 3.1 kb NcoI-NotI DNA fragment from pTK3 was inserted, containing a deletion in phoB. Apr, Tcr. | This study |
pYQ1 trpF | Derivative of pYQ1 in which a 0.7 kb NdeI-NotI PCR fragment encoding TrpF was inserted. Amr. | This study |
pYQ1 trpD | Derivative of pYQ1 in which a 1.1 kb NdeI-NotI PCR fragment encoding TrpD was inserted. Amr. | This study |
pYQ1 trpDC | Derivative of pYQ1 in which a 1.9 kb NdeI-NotI PCR fragment encoding TrpDC was inserted. Amr. | This study |
pYQ1 purH | Derivative of pYQ1 in which a 1.6 kb NdeI-NotI PCR fragment encoding PurH was inserted. Amr. | This study |
pYQ1 purE | Derivative of pYQ1 in which a 0.5 kb NdeI-NotI PCR fragment encoding PurE was inserted. Amr. | This study |
pYQ1 ilvD | Derivative of pYQ1 in which a 2.1 kb NdeI-NotI PCR fragment encoding IlvD was inserted. Amr. | This study |
pYQ1 aceEI | Derivative of pYQ1 in which a 2.7 kb NdeI-NotI PCR fragment encoding AceE1 was inserted. Amr. | This study |
pYQ1 PFLU3030 | Derivative of pYQ1 in which a 1.0 kb NdeI-NotI PCR fragment encoding PFLU3030 was inserted. Amr. | This study |
pYQ1 PFLU3887 | Derivative of pYQ1 in which a 1.0 kb NdeI-NotI PCR fragment encoding PFLU3887 was inserted. Amr. | This study |
pYQ1 PFLU5567 | Derivative of pYQ1 in which a 1.2 kb NdeI-NotI PCR fragment encoding PFLU5567 was inserted. Amr. | This study |
pYQ1 dsbC | Derivative of pYQ1 in which a 0.9 kb NdeI-NotI PCR fragment encoding DsbC was inserted. Amr. | This study |
pYQ1 sohB | Derivative of pYQ1 in which a 1.1 kb NdeI-NotI PCR fragment encoding SohB was inserted. Amr. | This study |
pYQ1 nagZ | Derivative of pYQ1 in which a 1.1 kb NdeI-NotI PCR fragment encoding NagZ was inserted. Amr. | This study |
pYQ1 anmK | Derivative of pYQ1 in which a 1.5 kb NdeI-NotI PCR fragment encoding AnmK was inserted. Amr. | This study |
pYQ1 ispA | Derivative of pYQ1 in which a 0.9 kb NdeI-NotI PCR fragment encoding IspA was inserted. Amr. | This study |
pYQ1 cbrB | Derivative of pYQ1 in which a 1.4 kb NdeI-NotI PCR fragment encoding CbrB was inserted. Amr. | This study |