Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosine

BMC Genomics

Table 1 Quantitative output from highly multiplexed targeted BS MiSeq run

Chromosome	Covered region	Length of the covered region (bp)	Number of CpG sites detected in covered region	Coverage full data set	Number of primer pairs
Chr4	46,391,588–46,392,165	578	44	5407	10
Chr3	11,034,244–11,035,166	923	90	19,607	7
Chr7	44,349,021–44,349,856	836	62	21,495	8
Chr11	31,820,209–31,820,665	457	26	1799	8
Chr11	31,825,606–31,827,393	1788	110	8232	16
Chr14	21,542,358–21,543,245	888	29	194	8
Chr14	21,558,269–21,559,093	825	49	3117	7
Chr17	35,299,547–35,300,293	747	61	1365	10
Chr19	42,489,794–42,490,445	652	16	5089	7
Chr20	61,992,854–61,993,598	745	53	1830	7
Chr2	162,284,277–162,284,993	717	58	369	7
Chr5	76,925,266–76,926,368	1103	104	12,744	8

ISSN: 1471-2164