Fig. 7

Histone modifications differentially affect the reproductive capacity of quiescent cells. a. Wild type cells (WT) and cells with mutations that abolish H2B ubiquitylation (htb-K123R), H3K4 methylation (hht-K4A), H3K36 methylation (hht-K36A), or H3K79 methylation (hht-K79A) were cultured in glucose-containing rich medium (YPD). Samples were removed from each culture at the diauxic shift (day 0) and at various times after glucose exhaustion for up to 30 days before spreading aliquots in triplicate onto YPD plates. The results are representative of a single experiment, and show the percentage of colony forming units relative to those at day 0, which was set as 100%. b Separation of wild type and hht-K79A cells on a Percoll gradient 7 days after inoculation into rich medium. c Relative proportions of quiescent and nonquiescent cells in 7-day cultures of wild type and hht-K79A strains. d Survival of wild type and hht-K79A quiescent cells incubated in water. The data represent the average with STD from two independent experiments. NQ, non-quiescent cell population; Q, quiescent cell population