Fig. 4

a NF-κB signaling canonical pathway diagram. This pathway was significantly enriched in the set of genes regulated by the high-protein diet (HPD). The diagram was obtained from Ingenuity Pathway Analysis software and depicts genes implicated in this pathway and their interactions. Expression of genes colored in green and red were respectively downregulated (q < 0.1) and upregulated (q < 0.1) in colonocytes of rats fed an HPD when compared to a normal-protein diet (NPD). The list of differentially expressed genes implicated in this pathway is presented in Additional file 6: Table S3. b Relative expression values of a selection of significantly differentially expressed genes (q < 0.01) participating to the NF-κB signaling pathway in the colonocytes of rats fed an HPD when compared to an NPD. The expression values were obtained by microarray experiment and normalized to the mean value in the NPD group. Nfkbie (NF-κB inhibitor epsilon), Rela (RELA proto-oncogene, NF-κB subunit), Nfkb1 and 2 (NF-κB subunit 1 and 2), Traf2 and 6 (TNF receptor associated factor 2 and 6). Data presented on histograms are means +/- S.E.M