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Fig. 6 | BMC Genomics

Fig. 6

From: Epithelial response to a high-protein diet in rat colon

Fig. 6

a Relative expression values of a selection of significantly differentially expressed genes (q < 0.01) related to proliferation in the colonocytes of rats fed a high-protein diet (HPD) when compared to a normal-protein diet (NPD). The expression values were obtained by microarray experiment and normalized to the mean value in the NPD group. Tfrc (transferrin receptor), Mt1a, 2a, 3 and 4 (metallothionein 1A, 2A, 3 and 4), Ppp2r2a (protein phosphatase 2 regulatory subunit B alpha), Ndrg1 (N-myc downstream regulated 1), Prdx1 (peroxiredoxin 1). b Staining of Ki67 by immunohistochemistry on distal colon of rats fed an NPD or an HPD. Ki67 labelling index was calculated as the percentage of Ki67 positive cells relative to the total number of cells within the same crypts. c Proliferating cell nuclear antigen (PCNA) protein expression was quantified by western blot in colonocytes of rats fed an HPD or an NPD. Band intensity was quantified and normalized to the intensity of the band corresponding to actin. b-c Mean values were compared with a t test. a-c Data presented on histograms are means +/- S.E.M

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