Fig. 7
From: Epithelial response to a high-protein diet in rat colon
a Heatmap representing the expression values of mucin genes in colonocytes (Muc). Each row corresponds to one Muc gene. Each column corresponds to a single rat fed a normal-protein diet (NPD), or a high-protein diet (HPD). The color indicates the relative expression value (as indicated by the key) obtained from microarray experiment and normalized to the mean value in the NPD group. Mean expression values of the HPD and the NPD groups were compared with a t test. *: p < 0.05. b Relative expression values of defensin genes significantly differentially expressed (q < 0.01) in the colonocytes of rats fed a HPD when compared to a NPD. The expression values were obtained by microarray experiment and normalized to the mean value in the NPD group. Defb3, 4, 10, 15, 19, 22 and 30 (β-defensin 3, 4, 10, 15, 19, 22 and 30). c Claudin 1 protein expression was assessed by western blot in colonocytes of rats fed an HPD or an NPD. Band intensity was quantified and normalized to the intensity of the band corresponding to actin. e-f Barrier function was evaluated with Ussing-chambers in distal colon of rats fed an NPD or an HDP. e Transmural resistance was measured for 15 min after mucosa mounting in the chamber. f FITC-dextran (FD4) transport from mucosal to serosal side was recorded during two hours and FD4 apparent permeability (FD4 Papp) was calculated. c-e For each parameter, mean values were compared with a t test. b-e Data presented on histograms are means +/- S.E.M