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Table 2 Summary of sequence assembly after Illumina sequencing

From: Transcriptome profile of rat genes in injured spinal cord at different stages by RNA-sequencing

Sample name

Raw reads

Clean reads

clean bases

Error rate (%)

Q20 (%)

Q30 (%)

GC content (%)

A1

44881934

40093612

6.01G

0.02

95.33

88.31

50.28

A2

54272034

45508458

6.83G

0.02

97.31

92.22

50.48

A3

46199060

41913544

6.29G

0.02

96.3

90.38

49.91

B1

52270362

44613686

6.69G

0.02

97.09

91.43

50.31

B2

47612764

43741790

6.56G

0.02

96.03

89.63

50.16

B3

58619478

42207044

6.33G

0.02

98.35

93.93

50.37

C1

47746200

44041564

6.61G

0.02

96.17

89.9

50.37

C2

52818270

45368646

6.81G

0.02

97.14

91.56

50.88

C3

41724966

40598352

6.09G

0.03

94.79

87.81

50.96

D1

54673906

45455460

6.82G

0.02

96.48

89.84

50.48

D2

41816080

40410590

6.06G

0.03

94.79

87.89

50.6

D3

45601476

41583286

6.24G

0.02

95.94

89.46

50.51

  1. A1- A3: sham; B1- B3: 1 dpi; C1- C3: 6 dpi; D1- D3: 28 dpi;
  2. Q20: The percentage of bases with a Phred value > 20;
  3. Q30: The percentage of bases with a Phred value > 30