Fig. 2

The pattern of Groucho recruitment to chromatin differs from that of the corepressor dCtBP. a Distribution of Gro and dCtBP binding site size. As indicated by a box plot, Groucho sites exhibit median widths of between 500 and 750 basepairs at the three time points sampled, although significantly larger peaks were also identified. dCtBP binding sites exhibits a median width of about 1000 bp. Thus, the difference between the short-range repression mediated by dCtBP and the long-range repression mediated by Gro cannot be attributed to one-dimensional spreading along chromatin. b Distribution of Gro and dCtBP binding sites relative to the nearest transcriptional start site (TSS). Gro ChIP-seq read density is enriched around TSS’s compared to dCtBP, which exhibits a small asymmetric depletion in these areas. c Distribution of Gro and dCtBP binding sites with respect to gene feature. Compared to dCtBP, Gro more often binds in intergenic regions, and therefore less frequently within either intronic or exonic regions