Fig. 6

Semi-quantitative RT-PCR validation of representative DEGs. a Dlx1 and Gdpd3 were specifically expressed in Sst-neurons. b Increased expression of Ppap2b, Pld1 and Crls1 are shown in nicotine treated Sst-neurons. c Nicotine increases the expression of Flt1, and decreases that of Nme4 and Entpd3 in Thy1-neurons. d The densitometry analysis of the RT-PCR results from a, b & c. The expression levels of corresponding genes were normalized by β-actin. Data are shown in means ± SEM from at least three independent experiments (*P < 0.05, **P < 0.001, Student’s t-test)