Fig. 2
Split of the cox2 gene in mtDNA of Campsomeris. Circular mitogenomes are arbitrarily linearized at the 3’ end of trnN. The Drosophila mtDNA represents the plesiomorphic mitogenome of Pancrustacea. The inverted mtDNA segment in Scolia (KT276222) and Campsomeris (KT740996, KX090217) is flanked by the trnD gene and the control region (CR). The inversion might have occurred due to recombination between similar and oppositely oriented trnK and trnQ genes. Other modifications of the C. p. fossulana mitogenome include single-gene inversions of trnQ, trnC and trnS1, translocations of trnF and trnL2, shuffling of trnS1, duplication of trnM, the presence of trnH CAT in addition to trnH CAC, the presence of trnK AAA (within the CR) in addition to trnK AAG, and the loss of trnI (or its replacement by a putative trnI gene located within rrnL). TrnS1, trnR and putative trnI encode tRNAs that lack the TψC (T) arm. The chromatopherogram of the cDNA sequence corresponding to the cox2a mRNA 3’ end (RACE product) shows that the cox2a stop codon, UAA, is generated by the polyadenylation of U (corresponding to T6195; KT740996). The positions of genes/ORFs in Campsomeris sp. HA10513 were deduced from comparison with the corresponding regions of C. p. fossulana mtDNA (5’ part of the reading frame of Campsomeris sp. HA10513 orf3, marked dark gray, is shifted in comparison to the reading frame of C. p. fossulana orf3). C. p. fossulana orf3-9 correspond to polyadenylated mRNAs that have been mapped by RACE. H2, N2, and K denote trnH CAT, putative (low covariance score) trnN AAT and trnK AAA (Campsomeris sp. HA10513) or trnK AAG (C. p. fossulana), respectively