Fig. 1
From: Lung function associated gene Integrator Complex subunit 12 regulates protein synthesis pathways
Optimizing INTS12 knockdown and elucidating its effect on snRNA processing in HBECs. a Optimizing anti-INTS12 D-siRNA transfections. INTS12 mRNA expression in HBECs transfected with three distinct D-siRNAs at 10nM (left) and with the indicated concentrations of D-siRNA A and C (right). D-siRNAs A and C at a concentration of 1nM were chosen for subsequent experiments. Statistical tests were performed comparing to scrambled D-siRNA control: *P < 0.05, **P < 0.01, ***P < 0.001. Individual ∆∆Ct gene expressions are GAPDH normalized and relative to the mean of scrambled D-siRNA condition. b Representative images of INTS12 protein expression in anti-INTS12 D-siRNA transfected HBECs by immunofluorescence. c INTS12 mRNA expression in HBECs transfected with D-siRNA A and C (left) and corresponding fold changes in misprocessed snRNAs (right). Statistical tests were performed comparing to scrambled D-siRNA control: *P < 0.05, ****P < 0.0001. Individual ∆∆Ct gene expressions are GAPDH normalized and relative to the mean of scrambled D-siRNA condition