High-throughput sequencing of pituitary and hypothalamic microRNA transcriptome associated with high rate of egg production

Table 4 Effect of SNPs on miRNA precursor energy changes

Precursor	SNP position in Precursor	SNP	SNP location	ΔΔG (kcal/mol)
gga-mir-1684a	6	C→G	stem	−1.9
	20	G→U	stem	5.0
	38	C→U	anti-stem	1.3
	39	A→G	anti-stem	−0.2
	43	U→C	loop	−2.7
	78	A→G	mature	−2.8
	78	A→C	mature	−6.9
gga-miR-1434	19	A→G	mature	0
	71	G→A	anti-stem	−0.1
	71	G→C	anti-stem	4.3

MFE minimum free energy
ΔΔGS the MFE difference value between wild-type precursors and SNP-type precursors. The minus value indicated that the SNP-type precursors had lower structure energy than the wild precursors. Otherwise, the former had higher structure energy than the latter

ISSN: 1471-2164