Fig. 9

GEX1A affected the splicing of PP2C and SnRK2 genes differently. The cDNAs were prepared from one-week-old Arabidopsis seedlings treated with 5 μM GEX1A or 25 μM ABA for 6 h, with DMSO as control. RT-PCR was performed using primers flanking the first and last exon of each gene. ”D” indicates DMSO treatment, “G” indicates 5 μM GEX1A treatment and “A” indicates 25 μM ABA treatment; gene names are indicated at the bottom of each panel. Functional transcripts of most of PP2C genes were removed by strong intron retention in GEX1A-treated plants, whereas under the same conditions, SnRK2.2, SnRK2.3, and SnRK2.6 kept producing functional transcripts with varying levels of intron retention. ABA did not cause obvious intron retention in the selected genes, when compared with DMSO treatment