Fig. 3

Distribution of selected mass shifts across amino acids. The height of bars within each row represents the fraction of total AA positions for the mass shift (indicated on the y axis) that were identified on each amino acid residue type (columns). Individual bars within each column represent fractions for each biological replicate (replicates 1, 2 and 3 from left to right within each column). Mass shifts are ordered by the single-AA bias score (the ratio of counts for the most commonly modified AA type to the mean of the counts for all other types; see Methods) with the highest score (most biased for a single AA) at the top; only the top 20 mass shifts are shown. Note that a constant mass shift of +57 Da was added to all cysteine modifications to correct for the presence of carbamidomethylation, meaning that a small number of cysteine modifications (e.g. +209 Da) fall outside of the mass range scanned by MODa (+/−200 Da)