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Table 3 The expression patterns of selected genes using real-time quantitative RT-PCR and RNA-Seq

From: Transcriptomic profiling of two Pak Choi varieties with contrasting anthocyanin contents provides an insight into structural and regulatory genes in anthocyanin biosynthetic pathway

Gene

RT-PCR

RNA-Seq (FPKM)

Gene annotation

 

‘G’

‘P’

‘G’

‘P’

 

Bra013652

0.88 ± 0.16 b

179.31 ± 87.33 a

79.26

11936.77

Leucoanthocyanidin dioxygenase

Bra019350

1.00 ± 0.05 b

2.72 ± 0.41 a

23.62

4969.47

Leucoanthocyanidin dioxygenase

Bra027457

1.35 ± 0.47 b

23.61 ± 2.38 a

67.91

15507.10

Dihydroflavonol-4-reductase

Bra037747

1.13 ± 0.11 b

3.08 ± 0.47 a

0

26.11

Flavonol synthase/flavanone 3-hydroxylase

Bra017520

1.18 ± 0.31 b

14.76 ± 3.48 a

0.94

21.80

Basic helix-loop-helix (bHLH) family protein

Bra017523

1.11 ± 0.14 b

28.85 ± 0.80 a

16.31

540.00

Basic helix-loop-helix (bHLH) family protein

Bra026967

1.12 ± 0.14 b

8.88 ± 1.45 a

0

12.00

oxidoreductase family protein

Bra016164

0.93 ± 0.11 b

193.23 ± 8.24 a

5.87

865.19

MYB08 Myb-related protein 308

Bra032635

0.01 ± 0.00 b

1.04 ± 0.03 a

2.84

373.93

ETC1 (ENHANCER OF TRY AND CPC 1); DNA binding / transcription factor

  1. The relative quantitation of gene expression was conducted via the 2 − ΔΔCt method, with actin as an endogenous reference. Data from three biological replicates were used to calculate the mean and standard deviation in DPS based on Student’s t-test. Values followed by different letters indicate significant difference at P < 0.05. ‘G’: Green-leafed variety; ‘P’: Purple-leafed variety