Fig. 1

Transcriptional Analysis of Fe repressed sRNA NrrF. Iron regulation was confirmed with real time PCR transcriptional analysis of RNA samples isolated for RNA-seq from cells collected 1,2,3,4 and 5 h. a The TSS was resolved by primer extension using a FAM labeled primer yielding a 123 bp fragment as analyzed with Peak Scanner b) and a transcript length of 154 bp as determined by Northern blot analysis d). TSS for NrrF is indicated by an ^ in the NrrF sequence in c), with red denoting the FB sequence and bold type the −10 promoter region. Blue represents the RIT, with the unlined portion of the sequence the location of the FAM labeled primer used for the primer extension c)