Fig. 2
Dcr2 is not involved in mRNA 3’ end processing a Dcr2 depletion does not affect CCC component protein levels. RNAi-depleted proteins are listed above the blot. Antibodies used for WB are listed to the left. b Dcr2 RNAi-depletion does not cause mRNA 3’ end misprocessing. An S1 nuclease assay was used to map histone (H)2A 3’ ends (left). Knockdowns are shown at the top. Potential mRNA 3’ end products are shown to the left: RT is the read-through misprocessed product, the open arrow marks the region of other misprocessed products, and the black arrow defines the properly processed product. CPSF73 is shown as a positive control. c RT-qPCR using primers that amplify misprocessed sop mRNAs (right) reveals very little misprocessed sop in Dcr2 knockdown samples. Knockdowns are shown on the x-axis. Degree of Misprocessing = Log10 (2^ΔΔCt(ORF-MP)). Error bars represent one standard deviation. d Dcr2 RNAi-depletion does not cause 3’ end misprocessing and read-through transcription. RNA-seq reads mapping to and downstream of the IP3K1 gene are shown in red. Maximal read counts for IP3K1 are shown on the y-axis