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Fig. 7 | BMC Genomics

Fig. 7

From: Drosophila melanogaster retrotransposon and inverted repeat-derived endogenous siRNAs are differentially processed in distinct cellular locations

Fig. 7

RetroTns dsRNA precursors are nuclear retained. RT-qPCR of retroTn and Esi1/2 mRNAs isolated from refined nuclear and cytoplasmic fractions reveals nuclear retention of retroTn esiRNAs precursors. (a, left) RetroTn RT-qPCR targets are shown on the x-axis. Fold change is the average of three experiments and is calculated as 2(Ct(Nuclear)-Ct(Cytoplasm)). (a, Right) CG44774 is the Esi1 precursor. CR18854 is the Esi2 precursor mRNA. GAPDH is the control transcript. Error bars represent one standard deviation. b Taqman qPCR of retroTn and Esi1/2 derived esiRNAs isolated from refined nuclear and cytoplasmic fractions shows nuclear retention of retroTn derived esiRNAs. Labels, calculations, and error bars are as in a. c RT-qPCR of esiRNA precursors in Symplekin, CPSF73 or Dcr2 knockdowns shows nuclear retention of both hp and retroTn dsRNA precursors. Labels, calculations, and error bars are as in a. RNAi-depletion is defined as in Fig. 4

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BMC Genomics

ISSN: 1471-2164

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