Table 1 Proteins differentially abundant during neural differentiation of ES cells. Fold change refers to increased levels (positive FC) or decreased levels (negative FC) of protein content from ES to D6 and D9. Fold change ± 1.5 (ANOVA p < 0.05) was used as threshold for protein identification
Spot # | Protein [Mus musculus] | Gene | Mw(kDa) | pI | SC(%) | FC | Array* |
|---|---|---|---|---|---|---|---|
297 | Inner membrane protein, mitochondrial | Immt | 80.9 | 6.8 | 62 | +1.8 | ↓b |
341 | 78 kDa glucose-regulated protein precursor | Hspa5 | 72.5 | 5.1 | 45 | +1.6 | -- |
367 | Far upstream element-binding protein 1 | Fubp1 | 67.3 | 7.2 | 61 | +2.1 | ↑a |
370 | Heat shock cognate 71 kDa protein | Hspa8 | 42.4 | 6.7 | 62 | −1.6 | ↓a |
380; 383 | Lamin-B1c | Lmnb1 | 67.0 | 5.1 | 56 | +2.4; +2.8 | -- |
403 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | Sdha | 73.6 | 7.1 | 53 | +2.1 | -- |
503; 511 | 60 kDa heat shock protein, mitochondrialc | Hspd1 | 59.6 | 8.1 | 44 | −1.8; − 2.0 | -- |
545 | Protein disulfide-isomerase A3 | Pdia3 | 57.1 | 5.9 | 49 | +1.9 | -- |
555 | Protein disulfide-isomerase | P4hb/Pdia1 | 57.4 | 4.8 | 45 | +1.6 | -- |
621 | Heterogeneous nuclear ribonucleoprotein H | Hnrph1 | 47.9 | 6.1 | 46 | +1.9 | ↑a |
624 | ATP synthase, mitochondrial F1 complex, alpha subunit | Atp5a1 | 54.9 | 9.4 | 14 | +1.9 | -- |
669 | Enolase 1B | Eno1 | 47.5 | 6.4 | 62 | −1.8 | ↓a |
693 | Reticulocalbin-2 | Rcn2 | 37.3 | 4.3 | 38 | +2.4 | ↑a |
751 | Actin, cytoplasmic 1 | Actb | 42.1 | 5.3 | 45 | −1.7 | -- |
808 | Mitochondrial import receptor subunit TOM40 | Tomm40 | 38.3 | 7.6 | 58 | −1.9 | ↓a |
851 | mCG49244 | C9orf156 | 21.8 | 5.7 | 76 | +2.6 | -- |
963 | Glyceraldehyde-3-phosphate dehydrogenase | Gapdh | 36.1 | 8.4 | 15 | −2.0 | -- |
1041 | Voltage-dependent anion-selective channel protein 2 | Vdac2 | 32.3 | 7.4 | 65 | −1.6 | ↓a |
1050 | YL2 protein | C1qbp | 23.8 | 4.4 | 13 | −2.6 | ↓a |
1134 | Phosphoglycerate mutase 1 | Pgam1 | 28.9 | 6.7 | 77 | +1.8 | ↓b |
1201 | Heat shock protein beta-1 | Hspb1 | 23.1 | 6.1 | 26 | −11.0 | ↓a |
1209 | Glutathione S-transferase A4 | Gsta4 | 25.6 | 6.8 | 49 | −3.4 | ↓a |
1450 | Cytochrome b-5, type B | Cyb5b | 13.3 | 5.7 | 41 | −1.9 | -- |
1545 | Fatty acid binding protein 7, brain | Fabp7 | 15.2 | 5.1 | 87 | +7.6 | ↑a |
1557 | Cellular retinoic acid-binding protein I | CrabpI | 15.6 | 5.3 | 92 | +13.7 | ↑a |